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High production of butyric acid by

Chao Ma,Jianfa Ou,Matthew Miller,Sarah McFann,Xiaoguang (Margaret) Liu

《化学科学与工程前沿（英文）》 2015年第9卷第3期页码 369-375 doi: 10.1007/s11705-015-1525-3

摘要： The objective of this study was to improve the production of butyric acid by process optimization using the metabolically engineered mutant of (PAK-Em). First, the free-cell fermentation at pH 6.0 produced butyric acid with concentration of 38.44 g/L and yield of 0.42 g/g. Second, the immobilized-cell fermentations using fibrous-bed bioreactor (FBB) were run at pHs of 5.0, 5.5, 6.0, 6.5 and 7.0 to optimize fermentation process and improve the butyric acid production. It was found that the highest titer of butyric acid, 63.02 g/L, was achieved at pH 6.5. Finally, the metabolic flux balance analysis was performed to investigate the carbon rebalance in . The results show both gene manipulation and fermentation pH change redistribute carbon between biomass, acetic acid and butyric acid. This study demonstrated that high butyric acid production could be obtained by integrating metabolic engineering and fermentation process optimization.

关键词： Clostridium tyrobutyricum butyric acid production fermentation mutant pH flux balance analysis

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Flow, thermal, and vibration analysis using three dimensional finite element analysis for a flux reversal

B. VIDHYA,K. N. SRINIVAS

《能源前沿（英文）》 2016年第10卷第4期页码 424-440 doi: 10.1007/s11708-016-0423-9

摘要： This paper presents the simulation of major mechanical properties of a flux reversal generator (FRG) viz., computational fluid dynamic (CFD), thermal, and vibration. A three-dimensional finite element analysis (FEA) based CFD technique for finding the spread of pressure and air velocity in air regions of the FRG is described. The results of CFD are mainly obtained to fine tune the thermal analysis. Thus, in this focus, a flow analysis assisted thermal analysis is presented to predict the steady state temperature distribution inside FRG. The heat transfer coefficient of all the heat producing inner walls of the machine are evaluated from CFD analysis, which forms the main factor for the prediction of accurate heat distribution. The vibration analysis is illustrated. Major vibration sources such as mechanical, magnetic and applied loads are covered elaborately which consists of a 3D modal analysis to find the natural frequency of FRG, a 3D static stress analysis to predict the deformation of the stator, rotor and shaft for different speeds, and an unbalanced rotor harmonic analysis to find eccentricity of rotor to make sure that the vibration of the rotor is within the acceptable limits. Harmonic analysis such as sine sweep analysis to identify the range of speeds causing high vibrations and steady state vibration at a mode frequency of 1500 Hz is presented. The vibration analysis investigates the vibration of the FRG as a whole, which forms the contribution of this paper in the FRG literature.

关键词： flux reversal generator air velocity computation fluid dynamics thermal analysis vibration analysis finite element analysis

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Quantitative analysis of yield and soil water balance for summer maize on the piedmont of the North China

Jingjing WANG,Feng HUANG,Baoguo LI

《农业科学与工程前沿（英文）》 2015年第2卷第4期页码 295-310 doi: 10.15302/J-FASE-2015074

摘要： The North China Plain (NCP) is a major grain production area in China, but the current winter wheat-summer maize system has resulted in a large water deficit. This water-shortage necessitates the improvement of crop water productivity in the NCP. A crop water model, AquaCrop, was adopted to investigate yield and water productivity (WP) for rain-fed summer maize on the piedmont of the NCP. The data sets to calibrate and validate the model were obtained from a 3-year (2011–2013) field experiment conducted on the Yanshan piedmont of the NCP. The range of root mean square error (RMSE) between the simulated and measured biomass was 0.67–1.25 t·hm , and that of relative error (RE) was 9.4%–15.4%, the coefficient of determination ( ) ranged from 0.992 to 0.994. The RMSE between the simulated and measured soil water storage at depth of 0–100 cm ranged from 4.09 to 4.39 mm; and RE and in the range of 1.07%–1.20% and 0.880–0.997, respectively. The WP as measured by crop yield per unit evapotranspiration was 2.50–2.66 kg·m . The simulated impact of long-term climate (i.e., 1980–2010) and groundwater depth on crop yield and WP revealed that the higher yield and WP could be obtained in dry years in areas with capillary recharge from groundwater, and much lower values elsewhere. The simulation also suggested that supplementary irrigation in areas without capillary groundwater would not result in groundwater over-tapping since the precipitation can meet the water required by both maize and ecosystem, thus a beneficial outcome for both food and ecosystem security can be assured.

关键词： AquaCrop summer maize soil water balance water productivity

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Flow and mass balance analysis of eco-bio infiltration system

Marla C. MANIQUIZ, Lee-Hyung KIM, Soyoung LEE, Jiyeon CHOI

《环境科学与工程前沿（英文）》 2012年第6卷第5期页码 612-619 doi: 10.1007/s11783-012-0448-1

摘要： A structured stormwater infiltration system was developed and constructed at a university campus and monitoring of storm events was performed during a one-year operation period. The flow and pollutant mass balances were analyzed and the overall efficiency of the system was assessed. While significant positive correlations were observed among rainfall, runoff and discharge volume ( = 0.93-0.99; <0.05), there was no significant correlations existed between rainfall, runoff, discharge volume and pollutant load. The system was more effective in reducing the runoff volume by more than 50% for small storm events but the difference between the runoff and discharge volume was significant even with rainfall greater than 10 mm. Results showed that the pollutant reduction rates were higher compared to the runoff volume reduction. Average pollutant reduction rates were in the range of 72% to 90% with coefficient of variation between 0.10 and 0.46. Comparable with runoff reduction, the system was more effective in reducing the pollutant load for small storm events, in the range of 80% to 100% for rainfall between 0 and 10 mm; while 65% to 80% for rainfall between 10 and 20 mm. Among the pollutant parameters, particulate matters was highly reduced by the system achieving only a maximum of 25% discharge load even after the entire runoff was completely discharged. The findings have proven the capability of the system as a tool in stormwater management achieving both flow reduction and water quality improvement.

关键词： best management practice flow balance low impact development mass balance nonpoint source stormwater runoff

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Metabolic flux analysis on arachidonic acid fermentation

JIN Mingjie, HUANG He, ZHANG Kun, YAN Jie, GAO Zhen

《化学科学与工程前沿（英文）》 2007年第1卷第4期页码 421-426 doi: 10.1007/s11705-007-0077-6

摘要： The analysis of flux distributions in metabolic networks has become an important approach for understanding the fermentation characteristics of the process. A model of metabolic flux analysis of arachidonic acid (AA) synthesis in ME-1 was established and carbon flux distributions were estimated in different fermentation phases with different concentrations of N-source. During the exponential, decelerating and stationary phase, carbon fluxes to AA were 3.28%, 8.80% and 6.97%, respectively, with sufficient N-source broth based on the flux of glucose uptake, and those were increased to 3.95%, 19.21% and 39.29%, respectively, by regulating the shifts of carbon fluxes via fermentation with limited N-source broth and adding 0.05% NaNO at 96 h. Eventually AA yield was increased from 1.3 to 3.5 g · L. These results suggest a way to improve AA fermentation, that is, fermentation with limited N-source broth and adding low concentration N-source during the stationary phase.

关键词： AA different fermentation concentration N-source exponential metabolic

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Transient analysis of thermoelastic contact problem of disk brakes

Ali BELHOCINE, Mostefa BOUCHETARA

《机械工程前沿（英文）》 2013年第8卷第2期页码 150-159 doi: 10.1007/s11465-013-0266-6

摘要：

The main purpose of this study is to analyze the thermomechanical behavior of the dry contact between the brake disk and pads during the braking phase. The simulation strategy is based on computer code ANSYS11. The modeling of transient temperature in the disk is actually used to identify the factor of geometric design of the disk to install the ventilation system in vehicles. The thermal-structural analysis is then used with coupling to determine the deformation established and the Von Mises stresses in the disk, the contact pressure distribution in pads. The results are satisfactory compared to those found in the literature.

关键词： brake disks heat flux heat-transfer coefficient Von Mises stress contact pressure

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High heat flux thermal management through liquid metal driven with electromagnetic induction pump

《能源前沿（英文）》 2022年第16卷第3期页码 460-470 doi: 10.1007/s11708-022-0825-9

摘要： In this paper, a novel liquid metal-based minichannel heat dissipation method was developed for cooling electric devices with high heat flux. A high-performance electromagnetic induction pump driven by rotating permanent magnets is designed to achieve a pressure head of 160 kPa and a flow rate of 3.24 L/min, which could enable the liquid metal to remove the waste heat quickly. The liquid metal-based minichannel thermal management system was established and tested experimentally to investigate the pumping capacity and cooling performance. The results show that the liquid metal cooling system can dissipate heat flux up to 242 W/cm² with keeping the temperature rise of the heat source below 50°C. It could remarkably enhance the cooling performance by increasing the rotating speed of permanent magnets. Moreover, thermal contact resistance has a critical importance for the heat dissipation capacity. The liquid metal thermal grease is introduced to efficiently reduce the thermal contact resistance (a decrease of about 7.77 × 10⁻³ °C/W). This paper provides a powerful cooling strategy for thermal management of electric devices with large heat power and high heat flux.

关键词： high heat flux liquid metal electromagnetic pump minichannel heat sink thermal interface material

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Observation of bioturbation and hyporheic flux in streambeds

Jinxi SONG, Xunhong CHEN, Cheng CHENG,

《环境科学与工程前沿（英文）》 2010年第4卷第3期页码 340-348 doi: 10.1007/s11783-010-0233-y

摘要： In the Elkhorn River, burrows, tubes, and sediment mounds created by invertebrate bioturbation were observed in the exposed streambed and commonly concentrated on the fine-sediment patches, which consist of silt, clay, and organic matter. These invertebrate activities could loosen the thin layer of clogging sediments and result in an increase of pore size in the sediments, leading to greater vertical hydraulic conductivity of the streambed (). The measurements of the vertical hydraulic gradient across the submerged streambed show that vertical flux in the hyporheic zone can alter directions (upward versus downward) for two locations only a few meters apart. In situ permeameter tests show that streambed in the upper sediment layer is much higher than that in the lower sediment layer, and the calculated in the submerged streambed is consistently greater than that in the clogged sediments around the shorelines of the sand bars. Moreover, a phenomenon of gas bubble release at the water-sediment interface from the subsurface sediments was observed in the groundwater seepage zone where flow velocity is extremely small. The bursting of gas bubbles can potentially break the thin clogging layer of sediments and enhance the vertical hydraulic conductivity of the streambed.

关键词： invertebrate bioturbation clogging hyporheic exchange streambed the Elkhorn River

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Simulation of bubble column reactors using CFD coupled with a population balance model

Tiefeng WANG

《化学科学与工程前沿（英文）》 2011年第5卷第2期页码 162-172 doi: 10.1007/s11705-009-0267-5

摘要： Bubble columns are widely used in chemical and biochemical processes due to their excellent mass and heat transfer characteristics and simple construction. However, their fundamental hydrodynamic behaviors, which are essential for reactor scale-up and design, are still not fully understood. To develop design tools for engineering purposes, much research has been carried out in the area of computational fluid dynamics (CFD) modeling and simulation of gas-liquid flows. Due to the importance of the bubble behavior, the bubble size distribution must be considered in the CFD models. The population balance model (PBM) is an effective approach to predict the bubble size distribution, and great efforts have been made in recent years to couple the PBM into CFD simulations. This article gives a selective review of the modeling and simulation of bubble column reactors using CFD coupled with PBM. Bubble breakup and coalescence models due to different mechanisms are discussed. It is shown that the CFD-PBM coupled model with proper bubble breakup and coalescence models and interphase force formulations has the ability of predicting the complex hydrodynamics in different flow regimes and, thus, provides a unified description of both the homogeneous and heterogeneous regimes. Further study is needed to improve the models of bubble coalescence and breakup, turbulence modification in high gas holdup, and interphase forces of bubble swarms.

关键词： bubble column computational fluid dynamics bubble breakup and coalescence population balance model bubble size distribution

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Gut microbial balance and liver transplantation: alteration, management, and prediction

null

《医学前沿（英文）》 2018年第12卷第2期页码 123-129 doi: 10.1007/s11684-017-0563-2

摘要：

Liver transplantation is a conventional treatment for terminal stage liver diseases. However, several complications still hinder the survival rate. Intestinal barrier destruction is widely observed among patients receiving liver transplant and suffering from ischemia–reperfusion or rejection injuries because of the relationship between the intestine and the liver, both in anatomy and function. Importantly, the resulting alteration of gut microbiota aggravates graft dysfunctions during the process. This article reviews the research progress for gut microbial alterations and liver transplantation. Especially, this work also evaluates research on the management of gut microbial alteration and the prediction of possible injuries utilizing microbial alteration during liver transplantation. In addition, we propose possible directions for research on gut microbial alteration during liver transplantation and offer a hypothesis on the utilization of microbial alteration in liver transplantation. The aim is not only to predict perioperative injuries but also to function as a method of treatment or even inhibit the rejection of liver transplantation.

关键词： gut microbial balance liver transplantation ischemia–reperfusion acute rejection

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Nonlinear sealing force of a seawater balance valve used in an 11000-meter manned submersible

《机械工程前沿（英文）》 2023年第18卷第1期 doi: 10.1007/s11465-022-0726-y

摘要： Balance valve is a core component of the 11000-meter manned submersible “struggle,” and its sealing performance is crucial and challenging when the maximum pressure difference is 118 MPa. The increasing sealing force improves the sealing performance and increases the system’s energy consumption at the same time. A hybrid analytical–numerical–experimental (ANE) model is proposed to obtain the minimum sealing force, ensuring no leakage at the valve port and reducing energy consumption as much as possible. The effects of roundness error, environmental pressure, and materials on the minimum sealing force are considered in the ANE model. The basic form of minimum sealing force equations is established, and the remaining unknown coefficients of the equations are obtained by the finite element method (FEM). The accuracy of the equation is evaluated by comparing the independent FEM data to the equation data. Results of the comparison show good agreement, and the difference between the independent FEM data and equation data is within 3% when the environmental pressure is 0–118 MPa. Finally, the minimum sealing force equation is applied in a balance valve to be experimented using a deep-sea simulation device. The balance valve designed through the minimum sealing force equation is leak-free in the experiment. Thus, the minimum sealing force equation is suitable for the ultrahigh pressure balance valve and has guiding significance for evaluating the sealing performance of ultrahigh pressure balance valves.

关键词： seawater balance valve sealing performance hybrid ANE model FEM minimum sealing force equation

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Mass and heat balance calculations and economic evaluation of an innovative biomass pyrolysis project

Quanyuan WEI, Yongshui QU, Tianwei TAN

《化学科学与工程前沿（英文）》 2011年第5卷第3期页码 355-361 doi: 10.1007/s11705-010-0567-9

摘要： Biomass can be converted into flammable gas, charcoal, wood vinegar, wood tar oil and noncombustible materials with thermo-chemical pyrolysis reactions. Many factors influence these processes, such as the properties of the raw materials, and temperature control and these will affect the products that are produced. Based on the data from a straw pyrolysis demonstration project, the mass and heat balance of the biomass pyrolysis process were analyzed. The statistical product and service solutions (SPSS) statistical method was used to analyze the data which were monitored on-site. A cost-benefit analysis was then used to study the viability of commercializing the project. The analysis included net present value, internal rate of return and investment payback period. These results showed that the straw pyrolysis project has little risk, and will produce remarkable economic benefits.

关键词： mass balance heat balance biomass pyrolysis economic benefit

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Application of cold spraying for flux-free brazing of aluminium alloy 6060

Kirsten BOBZIN, Lidong ZHAO, Thomas SCHLAEFER, Thomas WARDA,

《机械工程前沿（英文）》 2010年第5卷第3期页码 256-260 doi: 10.1007/s11465-010-0095-9

摘要： In the present study, samples of aluminium alloy 6060 were coated by cold spraying with a powder of brazing alloy Al12Si. The influence of the process gas temperature on particle velocities and coating build-up was investigated. The coated samples were heat-treated in air and under argon atmosphere to investigate the wetting behaviour of the deposited Al12Si and the diffusion processes between Al12Si coatings and substrates. Coated samples were brazed flux-free under argon atmosphere by an induction heating system. The microstructure of the coated, heat-treated, and brazed samples was investigated. The shear strength of the brazed joints was determined. The results show that the brazing alloy Al12Si could be very well deposited on the substrate by cold spraying. The particle velocity increased with increasing process temperature. Correspondingly, the thickness of Al12Si coatings increased with increasing process temperature. The heat treatments showed that a very good metallurgical bond between the Al12Si coatings and the substrate could be realized by the deposition using cold spraying. The coated samples could be well brazed without fluxes. The coating thickness and overlap width influenced the shear strength of the brazed joints. The highest shear strength of brazed joints amounts to 80 MPa.

关键词： aluminium alloy flux-free brazing cold spraying shear strength

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Deviation correction strategy for the earth pressure balance shield based on shield–soil interactions

《机械工程前沿（英文）》 2022年第17卷第2期 doi: 10.1007/s11465-022-0676-4

摘要： The control system presently used in shield posture rectification is based on driver experience, which is marginally reliable. The study of the related theory is flawed. Therefore, a decision-making approach for the deviation correction trajectory and posture rectification load for an earth pressure balance (EPB) shield is proposed. A calculation model of posture rectification load of an EPB shield is developed by considering the interactions among the cutter head, shield shell, and ground. The additional position change during the shield attitude correction is highlighted. The posture rectification loads and shield behaviors results can be solved by the proposed method. The influences of the stratum distribution (i.e., bedrock height in the upper-soft and lower-hard strata) on shield behaviors and posture rectification loads are analyzed. Results indicated that the increase of pitch angle in the upper-soft and lower-hard strata causes a sharp rise in vertical displacement. The bedrock height increases the magnitudes of the required posture rectification moments when h_r/D > 0.5. For a tunnel with h_r/D ≤ 0.5, the variation of h_r/D has little effect on the posture rectification moments. Finally, the posture rectifying curves based on the theoretical model are compared with the target ones based on the double circular arc interpolation method. The required results can be obtained regardless of the soil–rock compound stratum distribution. The maximum rectification moment in the rock layer is almost 12.6 times that in the soil layer. Overall, this study provides a valuable reference for moment determination and the trajectory prediction of posture rectification in compound strata.

关键词： additional position change deviation correction trajectory earth pressure balance shield mechanical model posture rectification